"pyrimethamine and proguanil resistance-conferring key mutations in dihydrofolate reductase gene of plasmodium falciparum isolates from iran"

using mutation specific pcr assay and analysis of position 108 amino acid in dihydrofolate reductase-thymidilate synthase gene in 70 plasmodium falciparum isolates of malarial patients from southern areas of iran, showed 4 different situations: iwild type (108-ser) ii-mutant type (108-thr) iii-mutant type (108-asn) and iv-mixed wild and mutant type (108-ser+thr) with frequency of 58(82.8%), 2(2.9%), 8(11.4%) and 2(2.9%) respectively. many studies have demonstrated that mutations in position 108 have key role for emerging resistance to anti-folate drugs in falciparum malaria so, the resistance to pyrimethamine and proguanil, presents in plasmodium falciparum isolates from malarious endemic regions of iran.